Journal: Wellcome Open Research
Article Title: Impact of shipping temperature on cell viability and T cell responses to bacterial antigens
doi: 10.12688/wellcomeopenres.18822.1
Figure Lengend Snippet: Influence of shipping temperature on cellular immune responses in PBMC samples obtained from recovered melioidosis patients one year after infection. ( A , B ) IFN-γ ELISpot responses to whole cell heat-inactivated Burkholderia pseudomallei (HIA-BP) and purified protein derivative (PPD). Each colored-dot represents one donor. N=7, Wilcoxon paired rank test. Statistical significance levels are indicated on the plot. ( C , D ) Spearman’s correlation between cell phenotype (% of live cells) and IFN-γ ELISpot response to BP ( C ) and PPD ( D ) respectively. The correlations were calculated using the fold-change difference between the two shipping temperatures (response at -196°C divided by response at -80°C). Each colored-dot represents each donor. Statistical significance levels are indicated on the plot. N=7, * p<0.05, ** p<0.01, *** p<0.001.
Article Snippet: Firstly, multiscreen-I 96 well filter plates (Milipore Cat#MAIPS4510) were coated with 10 ug/mL unconjugated anti-human IFN-γ mAb (MABTECH Cat# 3420-3-1000, RRID:AB_907282) diluted in autoclaved 0.05M carbonate bicarbonate buffer (Sigma-Aldrich Cat#C3041-100CAP) overnight at 4°C.
Techniques: Infection, Enzyme-linked Immunospot, Purification